Image Processing

Huygens STED Deconvolution Increases Signal-to-Noise and Image Resolution towards 22 nm

Vincent Schoonderwoerta1 c1, Remko Dijkstraa1, Grazvydas Luckinaviciusa2, Oliver Koblera3 and Hans van der Voorta1

a1 Scientific Volume Imaging bv, Laapersveld 63, 1213VB, Hilversum, The Netherlands

a2 Ecole Polytechnique Fédérale de Lausanne, Institute of Chemical Sciences and Engineering (ISIC), 1015 Lausanne, Switzerland

a3 Leibniz-Institute for Neurobiology, Combinatorial Neuroimaging (CNI), Brenneckestrasse 6, 39118 Magdeburg, Germany

Image restoration aims to make optimal use of the data provided by the light microscope to recover the object being imaged; in other words, to “figure out what the microscope is actually trying to tell us.” To achieve this, the distortions introduced by the imaging process need to be undone insofar possible.



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