Microscopy Today (2010), 18:8-14 Cambridge University Press

Copyright © Microscopy Society of America 2010


Feature Article

Atomic Force Microscopy Imaging of Living Cells

Alexandre Berquanda1 c1, Charles Roduita2, Sandor Kasasa2, Andreas Holloschia3, Leslie Poncea3 and Mathias Hafnera3a4


a1 Veeco Instruments GmbH, Dynamostrasse 19, 68165 Mannheim, Germany
a2 Institut de Physique des Systèmes Biologiques, Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
a3 University of Applied Sciences, Institute of Molecular and Cell Biology, Mannheim, Germany
a4 Medical Faculty Mannheim, University of Heidelberg, Mannheim, Germany

Over the last two decades, Atomic Force Microscopy (AFM) has emerged as the tool of choice to image living organisms in a near-physiological environment. Whereas fluorescence microscopy techniques allow labeling and tracking of components inside cells and the observation of dynamic processes, AFM is mainly a surface technique that can be operated on a wide range of substrates including biological samples. AFM enables extraction of topographical, mechanical and chemical information from these samples.


c1 aberquand@veeco.de

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